The FV3000 confocal microscope incorporates TruSpectral detectors that leverage proprietary spectral detection technology to achieve high sensitivity and spectral flexibility. This technology is capable of detecting even the faintest fluorophores and can achieve up to 3X more light transmission than traditional spectral detection technology by implementing the volume phase holographic (VPH) diffraction grating.

The confocal microscope's TruSpectral detectors have independently adjustable channels that allow for optimizing signal detection for each individual fluorophore. Moreover, the variable barrier filter mode enables simultaneous four-channel image acquisition with up to sixteen channels in sequential mode. Additionally, the Lambda scanning mode facilitates accurate spectral unmixing of complex overlapping fluorescent signals.

The FV3000 Red near-infrared (NIR) solution, with its carefully engineered NIR upgrades, extends the wavelength detection capabilities of the FV3000 microscope up to the NIR region of up to 890 nm. It is equipped with 730 nm or 785 nm lasers and an NIR-sensitive 1- or 2-channel GaAs detector, which can enable up to 6 channels for multiplexed imaging from violet to NIR (400 nm–890 nm). The TruFocus Red allows for stable and gentle NIR time-lapse imaging, and the X Line objectives offer the widest range of chromatic correction from 400 nm – 1000 nm for outstanding color accuracy during multicolor fluorescence imaging.

The FV3000 microscope offers both macro-to-micro imaging and super resolution microscopy, allowing users to acquire data and locate regions of interest for high-resolution imaging with ease.

• Users can begin by using a low-magnification 1.25X or 2X objective to quickly capture a large field of view (FOV) map of the whole specimen.
  

• After identifying regions of interest on the Overlay Map, users can switch to a higher magnification objective to perform high-resolution confocal imaging down to 120 nm with Olympus Super Resolution technology (FV-OSR). -

• Finally, users can finalize their acquisition and produce publication-ready microscope images with TruSight image processing.

The FV3000 Hybrid Scanner is a powerful tool for confocal imaging that offers two scanners in one, allowing for high-speed imaging and increased productivity.

The FV3000RS hybrid scan unit combines a galvanometer scanner and a resonant scanner, offering both precision scanning and high-speed imaging capabilities.

• The resonant scanner is ideal for capturing video-rate images with a large field of view, with speeds ranging from 30 frames per second (fps) at FN 18 up to 438 fps using clip scanning.

• The resonant scanner is also great for observing fast phenomena, such as a beating heart, blood flow, or calcium ion (Ca2+) dynamics inside cells.

• The hybrid scanner allows you to switch seamlessly between the galvanometer scanner and resonant scanner with the click of a button, making it easy to adapt to different imaging needs.

Time-lapse experiments require consistent focus and low phototoxicity to the sample.

• Olympus’ TruFocus unit helps maintain focus during live cell imaging despite changes in temperature or added reagents

• The FV3000 microscope’s high-sensitivity detector requires significantly less laser power while the resonant scanner reduces laser illumination time, lowering phototoxicity for more physiologically accurate confocal imaging data

The Sapphire imaher enhances your fluorescence imaging with stable signals, ensuring reliable quantitation for both weak and strong bands. With its ability to probe multiple proteins on a single blot, the Sapphire provides more results from every sample.

Get more research done in less time with higher sensitivity than film-based imaging techniques. The Sapphire’s chemiluminescence imaging is four times faster and more sensitive. It can detect down to femtogram amounts of your protein of interest.

The Sapphire offers improved flexibility with phosphor imaging, providing a wide dynamic range for sensitive detection and higher sensitivity, which are crucial when conducting phosphor imaging. Additionally, it can scan storage phosphor screens, eliminating the need for film-based autoradiography and providing more flexibility in your research.